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clindamycin hydrochloride Percutaneous absorption in the pig and the rat.

Number of visits:927 Date:2015/12/15 5:45:07
Percutaneous absorption of clindamycin hydrochloride in the pig and the rat.

clindamycin hydrochloride in a 3% formulation was applied twice daily to the back skin of three white (Yorkshire) and three black (Hampshire) pigs for 22 days. Partial thickness samples of skin were obtained by use of a dermatome on shaved opposite sides at 4 hours and 5 days posttreatment. More than twice the antibiotic activity (P less than or equal to .001) was found in thinner (254 micrometers epidermis) samples than in thicker (1016 micrometers epidermis and dermis, 1 to 3) samples. A significantly higher content of antibiotic (P = 0.015) was retained in the skin of black pigs at five days posttreatment. Groups of ten Upjohn Sprague-Dawley rats were treated similarly with clindamycin hydrochloride phosphate in a 3% formulation three times daily for 22 days. At termination of treatment antibiotic activity was widely distributed, whereas at five days posttreatment residual activity was confined largely to samples of treated skin. No cutaneous irritation due to treatment was observed in either study

In-vivo evaluation of clindamycin hydrochloride release from glyceryl monooleate-alginate microspheres by NIR spectroscopy

The purpose of this study was to use near-infrared (NIR) transmission spectroscopic technique to determine clindamycin hydrochloride plasma concentration after oral administration of clindamycin hydrochloride loaded GMO-alginate microspheres using rabbits as animal models. Lyophilized clindamycin hydrochloride–plasma standard samples at a concentration range of 0.001–1002μg/ml were prepared and analyzed by NIR and HPLC as a reference method. NIR calibration model was developed with partial least square (PLS) regression analysis. Then, a single dose in-vivo evaluation was carried out and clindamycin hydrochloride–plasma concentration was estimated by NIR. Over 2402h time period, the pharmacokinetic parameters of clindamycin hydrochloride were calculated for the clindamycin hydrochloride loaded GMO-alginate microspheres (F3) and alginate microspheres (F2), and compared with the plain drug (F1). PLS calibration model with 7-principal components (PC), and 8000–920002cmspectral range shows a good correlation between HPLC and NIR values with root mean square error of cross validation (RMSECV), root mean square error of prediction (RMSEP), and calibration coefficient () values of 0.245, 1.164, and 0.9753, respectively, which suggests that NIR transmission technique can be used for drug-plasma analysis without any extraction procedure. F3 microspheres exhibited controlled and prolonged absorptionof 4.0 vs. 1.0 and 0.502h;of 2.3702±020.3 vs. 3.8102±020.8 and 5.4302±020.702μg/ml for F2 and F1, respectively. These results suggest that the combination of GMO and alginate (1:4 w/w) could be successfully employed for once daily clindamycin hydrochloride microspheres formulation which confirmed by lowand highvalues.
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